Fig. 4.

Proteomic analysis reveals RC-APEX baits have unique interactomes that include known RC proteins. (A) Venn diagram showing the extent of overlap of the 55 high-confidence prey identified by the RC-APEX baits. High-confidence prey had a SAINT score ≥0.8, using SAINTexpress analysis. (B) Network map generated in Cytoscape of RC-APEX baits, indicated by colored squares, and their respective prey. The size of the prey gene circle was dependent on its abundance in terms of spectral counts compared with control samples [log₂(FoldChange]). Known RC proteins are outlined in black. Dotted lines indicate previously established interactions mined from MIST (Hu et al., 2018).