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. 2019 Jul 9;12(7):dmm037994. doi: 10.1242/dmm.037994

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — Effect of I_K,ACh blockade on heart rate (HR) in gnb5-knockout zebrafish. (A) Experimental setup for high-speed imaging of the zebrafish heart. HR was recorded at 150 fps for 10 s. (B) Time schedule of drug treatment and CCh challenge in gnb5-knockout larvae. (C) Scatter dot plots with mean of HR of gnb5-knockout larvae 5 days post-fertilization at basal level, after treatment with 50 µmol l⁻¹ XEN-R0703 or DMSO, and upon application of 500 µmol l⁻¹ CCh. DMSO, n=13; XEN-R0703, n=22. **P<0.01, ***P<0.001, ****P<0.0001 (two-way repeated measures ANOVA followed by Bonferroni corrected pairwise comparisons). (D) Percentage of gnb5-knockout larvae that responded to 500 µmol l⁻¹ CCh with a decrease in HR of 0-25, 26-50, 51-75 or 76-100%. DMSO, n=13; XEN-R0703, n=22. Only 27% of gnb5-knockout larvae pretreated with XEN-R0703 displayed a pathological decrease in HR (51-75%, 76-100%), while over 80% of the not-pretreated gnb5-knockout larvae display the same decreased level of HR.