Fig. 6.

Both Wnt1-Late^Ex tumor subpopulations have tumor-initiating potential. (A) Limiting dilution cell transplantation assay. Three primary tumors (two Wnt1-Early^Ex and one Wnt1-Late^Ex) were transplanted into five 6- to 8-week-old wild-type FVB female mice at each cell concentration and monitored for tumor growth over 120 days. (B) Cd49f/Epcam FACS profile of a representative Wnt1-Late^Ex CD49f^pos/Epcam^neg passaged tumor. (C) FACS population frequencies of Wnt1-Late^Ex CD49f^pos/Epcam^neg passaged tumors as measured from four independent transplanted tumors. Each measurement is represented as a cross. The error bars represent 1 s.d. (D) Cd49f/Epcam FACS profile of a representative Wnt1-Late^Ex CD49f^pos/Epcam^pos passaged tumor. (E) FACS population frequencies of Wnt1-Late^Ex CD49f^pos/Epcam^pos passaged tumors as measured from three independent transplanted tumors. Each measurement is represented as a cross. The error bars represent 1 s.d.