Skip to main content
. 2019 Jul 16;12(7):dmm038810. doi: 10.1242/dmm.038810

Fig. 6.

Fig. 6.

Silencing of IRS2 abolishes the activation of LX2 cells induced by IGF1. LX2 cells infected with scrambled (shC) or IRS2 shRNA (shIRS2) lentiviral particles were treated with 10 nM IGF1 for 24 h (A,B,D) or 15 min (C). (A) Representative phase-contrast images of LX2 cells. (B) Representative images of LX2 cells after immunofluorescence staining for COL1A1 and αSMA. Nuclei are stained blue with DAPI. (C) Representative western blots with the indicated antibodies (left) and their quantification (right). (D) Representative images of LX2 cells after immunofluorescence staining for MMP9. Nuclei are stained blue with DAPI. Statistical significance was assessed by one-way ANOVA and Bonferroni post-test: *P<0.05, IGF1 versus untreated cells; #P<0.05, shIRS2KO versus shC (n=4 independent experiments performed in duplicate). Scale bars: 100 μm (A) and 25 μm (B,D).