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. 2019 Jul 15;8(7):bio044362. doi: 10.1242/bio.044362

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — The effect of crizotinib on ALK phosphorylation of transgenic flies expressing hTPM4-hALK or hALK. Western blot analysis of the levels of total hALK and hALK phosphorylated at Tyr¹²⁷⁸ in flies overexpressing hTPM4-hALK or hALK. (A) hTPM4-hALK was phosphorylated at Tyr¹²⁷⁸ in a ligand-independent manner. The flies expressing hTPM4-hALK showed a decreased level of phosphorylated Tyr¹²⁷⁸ after crizotinib treatment compared with no treatment, with a greater decrease induced by 1 µM compared with 200 nM crizotinib. (B) Wild-type hALK was slightly phosphorylated. (C) Quantitation of the western blot results in A is represented graphically. NF, normal food.