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. 2019 Jul 8;8(7):bio044529. doi: 10.1242/bio.044529

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Chaperones can form OSFs under various stress conditions both in S. cerevisiae and O. parapolymorpha. (A) Cells of the BY4741 strain bearing the indicated GFP-fusion protein were grown in YP-Gly medium and then transferred onto medium with the indicated concentration of KCl, glycerol or sorbitol. (B) Cells bearing the indicated GFP-fusion chaperone were grown in YP-Gly or YPD medium to logarithmic phase and transferred onto the same medium supplemented with 1M KCl. (C) Cells of O. parapolymorpha, producing the closest Ssa1 homologue tagged with GFP (see Materials and Methods) were grown in YP-Gly medium to logarithmic phase and transferred onto YPD medium with 1M KCl. (D) Analysis of foci numbers in populations of cells used in B and C, with the vertical axis depicting the percentage of cells containing more than three foci. OP, O. parapolymorpha cells. Scale bars: 5 µm.