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. 2019 Jul 8;8(7):bio041673. doi: 10.1242/bio.041673

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Flow cytometry analysis of LGR5/E-cadherin, LGR5/CD26 and CD26/CD133 in sphere-derived cells. (A) Two representative dot plots of LGR5 versus E-cadherin expression in COLO205_sph and HT-29s_ph. (B) LGR5 versus CD26 expression in T84_sph. CD26^high/LGR5⁺cells are marked in R6 region in UR quadrant. Physical gatings (FCS versus SSC) of the four quadrants of T84_sph are shown. (C) Caco-2_sph representative dot plots for CD26 versus CD133 and, on the right, LGR5 versus E-cadherin dot plots of the four regions gated on the left CD26/CD133 dot plot.