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. 2019 Jul 19;20(14):3538. doi: 10.3390/ijms20143538

Figure 6.

Figure 6

Effect of AAL extract on the levels of hippocampal BDNF, pCREB, and pAkt proteins in scopolamine-induced cognitive deficit mouse brains. Representative images show DAB immunostaining for phospho-CREB (pCREB) in the hippocampal region (A). The immunostained positive cells were calculated in brain tissue sections from each mouse (B). The graphs show the number of pCREB-positive cells in the hippocampal region. Data are presented as the mean ± SEM (n = 3). ** p < 0.01 vs. NOR group, # p < 0.05 vs. SCO group. Hippocampus tissues were lysed and subjected to Western blotting with anti-BDNF, phospho-CREB, total CREB, phospho-Akt, and total Akt antibodies (C). The protein levels were normalized to total CREB, total Akt, and β-actin, respectively. NOR: normal control; SCO: scopolamine; AAL: A. atemoya leaf; TAC: tacrine.