Fig. 1.

Dynamic expression profiling of glucose metabolism-related genes in the liver of S. japonicum-infected mice. a Fasting blood glucose was detected from normal or S. japonicum-infected mice at indicated time points (0, 4, 7 and 11 weeks post-infection), and analyzed using two-way ANOVA. b Liver tissues were prepared from S. japonicum-infected mice at indicated time points (0, 4, 7 and 11 weeks post-infection). The mRNA levels of glycolysis-related important genes (Pdk1, Ldha, Glut4, Pkm2, Glut1, Pfkfb3, Aldoc, HK2, Eno3, Pfk) and gluconeogenesis gene (G6pc) in the liver of each group were evaluated by real-time PCR. The mRNA level of each gene was normalized to β-actin mRNA levels in each sample. c, d Immunofluorescence of Pho-AKT in the liver tissue from normal or S. japonicum-infected mice (0, 4, 7 and 11 weeks post-infection); original magnification, ×200. The mean optical density of Pho-AKT positive cells from 10 random fields in each mouse was digitized and analyzed using Image-Pro Plus software. Data are expressed as the mean ± SD of three independent experiments with 6 mice per group in each experiment (ANOVA/LSD: *P < 0.05, **P < 0.01, ***P < 0.001). Scale-bars: c, d, 50 μm