Fig. 8.

NCKX activity and functional mitochondria are required for melanosomal pigment production. (A–H) Confocal BF images of melan-a melanocytes incubated with corresponding compounds for 72 hours: DMSO (A); phenylthiourea (PTU, 200 nM) (B); DBZ (30 μM) (C); CGP-37157 (10 μM) (D); CCCP (2 μM) (E); Oligomycin (Oligo, 5 μM) (F); Rotenone (Rot, 0.4 μM) (G); and Leupeptin (Leu, 100 mM) (H). Scale bar: 10 µm. (I) Melanin quantification assays for melanocytes incubated with PTU (200 nM), DBZ (30 μM) and CGP-37157 (10 μM). Melanin content was assayed by measuring optical density at 492 nm (OD₄₉₂). Assays were performed in triplicate. (J) Melanin quantification assays for melanocytes incubated with PTU (200 nM); CCCP (2 μM); Oligo (5 μM); Rot (0.4 μM); Leu (100 mM). Melanin content was assayed by measuring optical density at 492 nm (OD₄₉₂). Assays in I and J were performed in triplicate. Data shown are means±s.e.m. of the number of triplicate experiments. n.s., not significant; *P<0.05; **P<0.01; ***P<0.001 (Student's t-test).