Figure. 1. NFKB1 in stroma is required for the growth of cancer.

A. Lack of p50^−/− in stromal cells leads to reduced growth of KPC pancreatic tumors. KPC pancreatic cancer cells were either injected alone (KPC) or co-injected with WT-PSC (KPC+WT-PSC) or p50^−/−-PSC (KPC+p50^−/− PSC) mice in the pancreas of WT-mice and allowed to grow for 30 days (n=at least 8 animals per group). *p<0.05. B. Pancreatic tumor sections harvested from different groups were stained with H&E or Sirius red (Collagen). IHC was performed for α-SMA and Vimentin. Quantification from 10 different fields of examination for 5 different mice is depicted. *p<0.05. C. Lack of NFκB in tumor stroma increases survival of mice with KPC pancreatic cancer. Mice with KPC, KPC+WT-PSC and KPC+p50^−/−-PSC tumors were followed until the study end-point was reached. Animal survival was plotted using Kaplan-Meir method. n=at least 10 in each group. D. Lack of p50 in stromal cells leads to reduced growth of another pancreatic cancer cell Panc02. Panc02 cells were either injected alone or co-injected with PSC isolated from WT or p50^−/− mice into the pancreas of WT-mice and followed for 30 days. n=at least 9 mice each group. *p<0.05. E. & F. The phenomenon that lack of NFKB1 in tumor stroma reduces tumor growth is observed in Melanoma and Lung cancer as well. E. Melanoma cell line B16-F10 (n=at least 6 mice in each group) or F. Lung cancer cell line LLC (n=at least 6 mice in each group) was injected, either alone, or with dermal or lung fibroblast respectively, from WT or p50^−/− mice subcutaneously in a WT-mice. The mice were sacrificed after 30 days and the tumor weights were compared. *p<0.05. See also Suppl. Figure 1