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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Mol Cancer Res. 2019 May 20;17(8):1710–1720. doi: 10.1158/1541-7786.MCR-18-1233

Figure 5: Knockdown of IDH1 increases repressive H3K9me2 at E2F gene target loci.

Figure 5:

Ovcar3 and Ovcar10 cells were infected with two independent hairpins targeting IDH1. Empty vector was used as a control.

(A) H3K9me2 ChIP was performed, and H3K9me2 binding to PCNA and MCM3 was determined by qPCR and is normalized to a gene desert region control. One of two experiments is shown. Data represent mean ± SD. *p<0.02

(B) RT-qPCR analysis PCNA and MCM3 expression. B2M was used as a reference gene. One of three experiments is shown. Data represent mean ± SD. *p<0.008

(C) Immunoblot analysis of PCNA and MCM3. Vinculin was used as a loading control. One of two experiments is shown.