Figure 4-. Knockdown of other EKC/KEOPS complex members does not produce the same TP53-dependent effects as TPRKB.

A) TPRKB is a member of the EKC/KEOPS complex, which includes the canonical proteins PRPK, OSGEP and LAGE3. To investigate the TP53 dependent effects of other EKC/KEOPS complex members on proliferation, PRPK, OSGEP or LAGE3 were knocked down using shRNA in H358 TP53^−/− LacZ and H358 TP53^WT cells as in Figure 3. B) Proliferation of benign immortalized MCF10A (TP53 wt) cells was similarly assessed after stable knockdown of the indicated EKC/KEOPS complex member by shRNA. All experiments utilized triplicate samples, with the average and standard error plotted.

* indicate p-values < 0.05 and ** indicate p-values <0.01.