A) Interactions between PRPK and TPRKB lead to TPRKB stabilization, while the presence of TP53 leads to reduced TPRKB levels. Additionally, TP53 is able to mediate TPRKB degradation through the proteasome, despite the fact that TPRKB and TP53 do not directly interact. Influences of these proteins may be key in phenotypes we witness. B) In cells that maintain wild-type TP53, loss of TPRKB does not have a profound effect on cell viability. In cells in which TP53 is perturbed, either through loss of wild-type protein, mutation of TP53, or disturbance of TP53 in some other manner (i.e. MDM2 amplification), TPRKB depletion leads to a reduction in cell proliferation.