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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Biol Lipids. 2019 Jun 5;1864(10):1514–1524. doi: 10.1016/j.bbalip.2019.05.017

Figure 5. Ceramide depletion induces translocation of Hsd17b4 to peroxisomes and interaction with Pex5.

Figure 5

A-C. HEK293T cells expressing the peroxisome marker Per-CFP (green) were depleted of ceramide by FB1 treatment and subjected to immunocytochemistry using antibody against Hsd17b4 (red). A, top view; B, side view. Arrows point at peroxisomes co-localized with Hsd17b4. C, Quantitative analysis (Pearson’s coefficient) for colocalization of Per-CFP with Hsd17b4. N=5, P<0.01. D, E. PLAs using antibodies against Pex5 (rabbit IgG) and Hsd17b4 (mouse IgG) with HEK293T cells expressing Per-CFP (green). E is quantification of D for number of PLA signals. N=4.