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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Eur J Immunol. 2019 May 2;49(8):1153–1166. doi: 10.1002/eji.201847931

Figure 6. SEMA7A blockade impairs conjugate formation between cytokine pre-activated NK cells.

Figure 6.

Control or cytokine (50 ng/mL IL-12+ 10 ng/mL IL-18+ 1 ng/mL IL-15) pre-activated CFSE-labeled and CellTrace Violet-stained autologous NK cells were co-cultured at 1:1 ratio in the presence of isotype control, anti-SEMA7A or anti-integrin-β1 antibodies and the formation of conjugates analyzed after 0 or 2 hours. Conjugates were identified as double positive cells by flow cytometry. (A) Flow cytometry plots of one representative experiment out of 2 with the percentage of conjugates indicated for each condition. (B) Bar graphs representing the numbers of conjugates formed at 2 hours for control and pre-activated NK cells in the presence of indicated antibodies.