Skip to main content
. 2019 Jul 22;2019:8576961. doi: 10.1155/2019/8576961

Figure 3.

Figure 3

OP drives apoptosis in NSCLC A549 cells by promoting mGlo2 association to Bax. (a) Mitochondrial lysates from A549 cells treated with 150 μM oleuropein (OP) were immunoprecipitated with protein G agarose-coupled anti-Glo2 (IP : Glo2) and subjected to western blotting (WB) with the anti-Bax antibody (WB : anti-Bax). Blots were then stripped and reprobed with the anti-Glo2 antibody to ensure equal immunoprecipitation of Glo2 protein. Mouse IgG was used as a negative control for immunoprecipitation; (b) mitochondrial lysates from A549 cells treated with 150 μM OP under SOD2 silencing (siSOD2) or control (siCtr, nonspecific siRNA) were immunoprecipitated with protein G agarose-coupled anti-Glo2 (IP : Glo2) and subjected to western blotting (WB) with the anti-Bax antibody (WB : anti-Bax).