Figure 5:

Intravenous administration of PLA particles to mice, as a single injection or as multiple daily injections, alters the ex vivo cytokine responses of splenocytes to LPS and CpG-ODN stimulation. (A) Schematic of dosing and experimental design to evaluate inflammatory cytokine secretions by splenocytes. (B) Cytokine secretions of splenocytes obtained from PLA-PVA or PLA-PEMA particle-treated mice that were stimulated ex vivo with LPS (100 ng/mL) or (C) CpG-ODN (100 ng/mL). Cytokines were measured by ELISA after 2 days of culture. Statistical differences were determined by one-way ANOVA with Tukey’s multiple comparisons test. In each panel, all groups were compared to each other and matching letters indicate no significant difference (p>0.05). For all non-matching letters, the groups were statistically different (p<0.05). Error bars represent standard deviation (n=3) and represent 2 independent experiments.