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Kinetic analyses of 4E-BP1, 4E-BP1 (R51–N64), and eIF4G peptides via SPR. The effect of temperature on (A) ka (B) kd and (C) kd. Statistical significance was determined using unpaired, two-tailed Student’s t tests. ns = not significant (p > 0.05). (A) ***: p = 0.0004. (B) ****: p = <0.0001; **: p = 0.0015. (C) *: p = 0.0162; ***: p = 0.0005; ****: p = <0.0001. Please see Tables S1–S3 for tabulated results, p values and statistical analyses.
