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. Author manuscript; available in PMC: 2020 May 23.
Published in final edited form as: J Med Chem. 2019 May 9;62(10):4967–4978. doi: 10.1021/acs.jmedchem.9b00068

Figure 7.

Figure 7.

Cellular inhibitory activities of the sTIP-04, HCS-4E-BP1, and HCS-4E-BP1 (LMAA) peptides as determined via m7GDP cap affinity chromatography. (A) Assay scheme. (B) Inhibition of the eIF4E–4E-BP1 and eIF4E–eIF4G PPIs in MDA-MB-231 cells. In all cases, cells were treated with peptides dissolved in water for 6 h and eIF4E was used as a normalization control. (C) Quantitation of the HCS-4E-BP1 data in (B) from triplicate biological replicates. (D) Quantitation of the sTIP-04 data in (B) from triplicate biological replicates. Please see Tables S9 and S10 for p values.