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Antiproliferative effects of (A) HCS-4E-BP1, (B) sTIP-04, and (C) HCS-4E-BP1 (LMAA) as determined via the CellTiter-Glo assay. Statistical significance was determined using unpaired, two-tailed Student’s t tests. ns = not significant (p > 0.05). (A) *: p = 0.0259; **: p = 0.0066; (B) ****: p = <0.0001. (D) Measurement of cell membrane damage by sTIP-04 (15 μM) using the LDH cytotoxicity assay using water and 1% Triton as negative and positive controls, respectively.
