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SPR Analysis of Linear and HCS-4E-BP1 and -eIF4G and sTIP-04 Peptides at 25 °C
| Peptide | Sequence | Ka×106 (M−1s−1) | kd×10(s−1) | Kd (nM) |
|---|---|---|---|---|
| 4E-BP1 |  |
0.82 ± 0.3 | 21 ±4 | 26 ±6 |
| HCS-4E-BP1 | 1.5 ± 0.2 | 5.3 ±4 | 4.1 ±3 | |
| eIF4G |  |
1.7 ± 0.3 | 48 ± 9 | 29 ±6 |
| HCS-eIF4G | 0.33 ± 0.01 | 30 ±4 | 90 ±0.4 | |
| Linear sTIP-04 |  |
2.0 ± 0.1 | 123 ± 14 | 62 ±6 |
| sTIP-04 | 0.83 ± 0.05 | 5.2 ± 0.1 | 6.3 ± 0.4 | |
