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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Exp Mol Pathol. 2019 May 11;109:42–50. doi: 10.1016/j.yexmp.2019.104261

FIG.2. Effects of HNG on Temozolomide (TMZ)-induced changes of cleaved PARP and phosphorylated STAT3 in liver and spleen.

FIG.2.

Protein expressions in liver and spleen were measured by Western blots and optical density ratio was used to compare protein expression changes between different groups. (A) Changes of cleaved PARP in liver tissue. Compared with control, HNG alone did not change cleaved PARP. HNG significantly attenuated TMZ-increased cleaved PARP. Uncleaved PARP was used as nomalizer. (B) Changes of phosphorylated STAT3 in liver tissue. Compared with control, HNG alone did not change phosphorylation of STAT3. HNG significantly attenuated TMZ-suppressed STAT3 phosphorylation. STAT3 was not different in any treatment group. (C) Changes of cleaved PARP in spleen tissue. Compared with control, HNG suppressed cleaved PARP in SCID mice with or without TMZ treatment. Uncleaved PARP was used as nomalizer. (D) Changes of phosphorylated STAT3 in spleen tissue. Compared with control, HNG increased phosphorylation of STAT3 in SCID mice with or without TMZ treatment. STAT3 was not different in any treatment group. Values are means ± SEM.