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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Exp Mol Pathol. 2019 May 11;109:42–50. doi: 10.1016/j.yexmp.2019.104261

FIG.4. Effect of HNG on Temozolomide (TMZ)-induced changes of STAT3, p38MAPK, BAX, and Cytochrome C in testis.

FIG.4.

Protein expressions in testis were measured by Western blots and optical density ratio was used to compare protein expression changes between different groups. (A) Compared with control, HNG alone did not change phosphorylation of STAT3. HNG significantly attenuated TMZ-suppressed STAT3 phosphorylation. STAT3 was not different in any treatment group. (B) Change of p38MAPK phosphorylation were detected by an activity kit and presented by optical density ratio of phosphorylated ATF2/ATF2 as described in methods. Compared with control, HNG alone did not change phosphorylation of p38MAPK. HNG co-treatment significantly suppressed TMZ-induced p38MAPK activation. (C) Compared with control, HNG alone did not affect BAX expression in the mitochondria (COX IV as a loading control for mitochondrial fraction). HNG co-treatment prevented TMZ-induced BAX increase in the mitochondria. (D) Compared with control, HNG alone did not change cytochrome c expression in the cytoplasmic fraction. HNG co-treatment partially prevented TMZ-induced increased in cytochrome c in the cytoplasm. Values are means ± SEM.