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. 2019 Aug 5;10(8):583. doi: 10.1038/s41419-019-1805-9

Fig. 3. TOPK inhibition increases the sensitivity of glioma cells to TMZ through induction of autophagy.

Fig. 3

a TOPK-silencing-Hs683 (top) or TOPK-silencing-H4 (bottom) cells were treated with TMZ (300 μM, 48 h) and wortmannin (5 μM, 24 h). Samples of whole cell lysates were analyzed using western blots. b Hs683 (top), H4 (bottom) cells were treated with OTS964 (1 μM, 48 h), TMZ (300 μM, 48 h), and wortmannin (5 μM, 24 h). Samples of whole cell lysates were analyzed by western blots using indicated antibodies. Data represent the results of triplicate experiments. c TOPK-silencing-Hs683 (left) or TOPK-silencing-H4 (right) cells were treated with TMZ and wortmannin in MTT assay. d Hs683 (top) or -H4 (bottom) cells were treated with TMZ and wortmannin in MTT assay. e TOPK-silencing-H4 cells were treated with TMZ and wortmannin in softagar assay (left). Scale bar: 100 μm. The numbers of clones were analyzed using Prism 5 software (right). The data were presented in the form of mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001. f H4 cells were treated with OTS964, TMZ, and wortmannin in softagar assay (left). Scale bar: 100 μm. The numbers of clones were analyzed using Prism 5 software (right). The data were presented in the form of mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001