Table 2.
Sequences primers for real-time PCR or ChIP-qPCR
| Genes | Forward primer | Reverse primer |
|---|---|---|
| Primers for real-time PCR | ||
| GAPDH | TGTTCGACAGTCAGCCGCAT | CGCCCAATACGACCAAATCCGT |
| RUNX2 | ACCACAAGTGCGGTGCAAAC | ACTGCTTGCAGCCTTAAATGACTCT |
| ALP | TAAGGACATCGCCTACCAGCTC | TCTTCCAGGTGTCAACGAGGT |
| OCN | CACCATGAGAGCCCTCACACTC | CCTGCTTGGACACAAAGGCTGC |
| PRMT3 | GGACATCCATGTGCACGGCAA | AGTGTGTTTTGGTGCTCTGAGGG |
| COL1A1 | CTGGCGCAGATGGTGTTGCT | TTCCAGTCAGACCCTTGGCAC |
| U6 | CTCGCTTCGGCAGCACA | AACGCTTCACGAATTTGCGT |
| miR-3648 | GCGAGCACAGAATTAATACGAC | AGCCGCGGGGATCGCCGAGGG |
| Primers for ChIP-qPCR | ||
| Primer-1 | GTGGTCTCTCGTCTTCTCCC | GTCGCACGAACGCCTGTC |
| Primer-2 | GCCGATCCTCTTCTTCCCC | GGGAGAAGACGAGAGACCAC |