FIGURE 2.
CC induce PTF1.2 dependent on the TF pathway. (A) PTF1.2 induction over time (60, 120, and 240 min) upon exposure to CC 2000 μg/ml, PBS/HSA, E. coli (1 × 107 particles/ml), or glass (n = 7 donors) in human whole blood. (B) Effect of TF inhibition following exposure of CC (500, 1000, and 2000 μg/ml) or E.coli after addition of the functional-grade inhibitory Ab against TF (anti-TF) or the corresponding Ultra-LEAF purified IgG1κ control Ab (Ctr IgG) for 240 min in human whole blood (n = 6 donors). (C) Effect of factor XII inhibition by CTI (40 μg/ml) in PFP following incubation with CC (2000 μg/ml) or glass for 60 min (n = 5). Effect of CC on platelet aggregation in human whole blood measured by electrical impedance aggregometry in (D) by CC (2000 μg/ml) or its controls, HSA/PBS, or TRAP (n = 6), (E) by additional stimulation with ADP (n = 3), and in (F) with TRAP following 6 min of incubation (n = 3), given as arbitrary units × min. In all experiments, thrombin was inhibited with lepirudin. All data are given as means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.