FIGURE 6.

Immunohistochemistry of frozen sections of human intracranial thrombus retracted from a patient with advanced carotid atherosclerosis and explored with polarization filter reflected light microscopy. (A and B) Staining against the monocyte marker CD14. (C) Staining against TF. (D) Staining against the complement neoepitopes C5b–9. (E) Staining against the complement neoepitope iC3b. The thicknesses of the sections are 4 μm. Pictures in the left column are taken by original magnification ×4 objective, and the CC-rich area (blue frame) is further augmented with an original magnification ×40 objective (middle and right columns). Examples of birefringent structures are shown by yellow arrows, whereas blue arrows point to the outer thrombi sections stained heavily for neoepitope iC3b. Scale bars, 200 μm for pictures in left column and 30 μm for pictures in middle and right columns.