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. 2019 Jun 24;203(4):922–935. doi: 10.4049/jimmunol.1900169

FIGURE 4.

FIGURE 4.

IL-36γ increased the expression of WNT5A in MDMs and inhibited H37Rv survival in MDMs. (A–F) qRT-PCR and Western blot analysis of WNT5A expression in MDMs treated with different levels of rhIL-36γ (A and B), with combination of rhIL-36γ and rhIL-36Ra and infected with H37Rv (C and D) or transfected with small interfering IL-36γ and then infected with H37Rv (E and F). (G) MDMs were pretreated with SB203580, U0126, SP600125, JSH-23, or LY294002, with DMSO as the solvent control for 1 h before infection with BCG in the presence of rhIL-36γ and above inhibitor for 48 h. WNT5A protein was analyzed by Western blot. (H–J) The numbers of viable intracellular bacteria in MDMs infected with H37Rv and treated with rhWNT5A (H), transfected with WNT5A siRNA (I), or combined with treatment of rhIL-36γ (J) were determined using CFU assays. Data presented are from one of least three independent experiments with similar results, and data are shown as means ± SD. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.