Skip to main content
. 2019 Jun 24;203(4):922–935. doi: 10.4049/jimmunol.1900169

FIGURE 7.

FIGURE 7.

WNT5A triggered autophagy via the COX-2/AKT/mTOR signaling pathway. (A and B) COX-2 expression in H37Rv-infected MDMs treated with or without rhWNT5A (A) or transfected with WNT5A siRNA or control siRNA (B) was detected by Western blot. (C) COX-2 expression in H37Rv-MDMs treated with rhWNT5A combined with or without BOX5 or XAV-939 was analyzed by Western blot. (D) LC3 conversion in MDMs transfected with COX-2 siRNA or control siRNA before infection with H37Rv and incubated rhWNT5A was analyzed by Western blot. (E) LC3 conversion in MDMs pretreated with NS-398, with DMSO as the solvent control before infection with H37Rv and incubated with combination of rhWNT5A and NS-398, was analyzed by Western blot. (F) Protein levels of COX-2, p-AKT/AKT, and p-mTOR/mTOR in MDMs infected with H37Rv and treated with rhWNT5A (left), or transfected with WNT5A siRNA (right), were measured by Western blot. All the samples of Western blot were collected and tested after 24 h of infection. Data presented are from one of least three independent experiments with similar results, and data are shown as means ± SD.