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. 2019 Jul 30;12:6083–6092. doi: 10.2147/OTT.S211560

Figure 4.

Figure 4

Inhibition of ATR promotes apoptosis induced by cisplatin. (A) PI analysis of cell cycle progression of SW1353 cells treated with the indicated concentrations of VE-822 either alone or in combination with 10 μl cisplatin for 24 h. (B) The quantitative data from A. (C) PI/FITC-Annexin V staining analysis of apoptosis of SW1353 cells treated with the indicated concentrations of VE-822 alone or in combination with 10 μM cisplatin for 24, 48, or 72 h. (D) The quantitative data from C. (E) PI staining analysis of cell death in SW1353 cells, empty vector (E.V.) transfected SW1353 cells, and ATR shRNA transfected cells that were cultured in the presence or absence of 10 μM cisplatin for 24 h. (F) The quantitative data from E. (G) Western blot analysis of the levels of cleaved caspase 3 in untreated SW1353 cells, cells transduced with empty vector (E.V.) or cells transduced with ATR shRNA, treated with 10 μM cisplatin for 24 h.