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. 2017 Dec 6;30(12):771–780. doi: 10.1093/protein/gzx059

Fig. 3.

Fig. 3

A comparison of direct fusion to EGFP with SpyoIPD/SpyTag labeling. Brightfield (DIC) and fluorescence (Fluor) images are shown for direct fusions of a target protein to EGFP (EGFP fusion) and for labeling of the target protein using the SpyoIPD/SpyTag strategy (SpyoIPD/ST). The identity of the target protein is given to the left of the images. The no SpyTag strain expresses SpyoIPD-EGFP, but no SpyTagged protein. CDC12: direct fusion to EGFP results in a distorted cell morphology in ~5% of CDC12-EGFP expressing cells.; HTB2: direct fusion to EGFP and the SpyoIPD/SpyTag strategy both show the expected labeling of the nucleus; PMA1: direct fusion to EGFP results in significant vacuolar mislocalization.