Fig. 6.

Using SpyoIPD-EGFP to study Pma1 temporal dynamics in single cells. Each trace in the plot corresponds to the total cellular fluorescence versus time for a single yeast cell, with t = 0 h corresponding to the time of glucose addition. Fluorescence decreases over time as SpyoIPD-EGFP is cleared from the trapped mother cell through cell division, SpyoIPD-EGFP turnover and Pma1 turnover. See Supplementary Information for a detailed description of how these data are analyzed to calculate the in-membrane half-life of fluorescently labeled Pma1. Inset: The posterior probability for the half-life of Pma1, ${\tau }_m$, found by integrating the probability corresponding to the surface in Fig.S5.