Figure EV2. IL‐7‐dependent proliferation of γδ17 T cells in pLNs of young mice.

Mice were treated with 4 mg/kg IgG2b or anti‐IL7 antibody by i.p. injection followed by administration of EdU by i.p. injection and supply in drinking water (as shown in Fig 5I). The level of proliferation was assayed by the level of EdU incorporation over a period of 3 days.

• A
  The proliferation of γδ1 and γδ17 T cells within each γδ T‐cell subsets under treatment with control isotype IgG2b or with anti‐IL‐7 neutralising antibody.
• B, C
  The proliferation of bulk CD4⁺ T cells and CD44^hi memory CD4⁺ T cells (B), as well as bulk CD8⁺ T cells and CD44^hi memory CD8⁺ T cells (C), under treatment with control isotype IgG2b or with anti‐IL‐7 neutralising antibody.

Data information: Results shown are collected from two independent experiments with 14 young mice (seven each for control and experimental groups). Statistical significances for changes in expression levels were assessed by two‐way ANOVA (A) or Mann–Whitney test (B and C). Error bars represent SD. *P < 0.05; ****P < 0.0001.