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. 2019 Jun 18;20(8):e47047. doi: 10.15252/embr.201847047

Figure 6. Kugeln do not interact with macrophages.

Figure 6

  1. The possible interaction of macrophages with kugeln was studied in the transgenic Tg(kdrl:HRAS‐mCherry) s916 , Tg(fms:GAL4.VP16) i186 , Tg(UAS‐E1b:nfsB.mCherry) il149, visualizing macrophages and the vasculature simultaneously.
  2. Coloured depth coding indicates the different anatomical depths of kugeln and macrophages (ventral purple, dorsal white).
  3. Maximum intensity projections of z‐stacks acquired every 10 min during a time‐lapse showed no interaction of macrophages (unfilled arrowhead) with kugeln (filled arrowhead; n = 21 kugeln in eight 3 dpf embryos). Macrophages are all white, indicating they are on a different z‐plane to the vessels seen.