Skip to main content
. 2019 Jun 18;20(8):e47047. doi: 10.15252/embr.201847047

Figure 8. Kugel number is decreased by Notch inhibition but increased by Wnt inhibition or activation.

Figure 8

  1. Kugel number was significantly decreased by inhibition of Notch signalling by 12 h treatment with 50 μM DAPT (****P < 0.0001; control n = 24 embryos 9.38 ± 1.77 (mean ± s.e.m.), DAPT n = 24 embryos 0.92 ± 0.28 (mean ± s.e.m.); 4 dpf; three experimental repeats; Mann–Whitney U‐test).
  2. Mean kugel diameter was not statistically significantly altered by DAPT treatment (P = 0.0832; control n = 225 kugeln from 24 embryos 8.64 ± 0.54 (mean ± s.e.m.); DAPT n = 22 kugeln from 24 embryos 6.88 ± 0.86 (mean ± s.e.m.); 4 dpf; three experimental repeats; Student's t‐test).
  3. A transgenic dll4 reporter line showed no indication of different dll4 expression at the sites of kugeln (white arrowheads pointing to kugeln; n = 122 kugeln from 23 3 dpf embryos; two experimental repeats).
  4. Studying Notch signalling in the Notch reporter line Tg(TP1glob:venusPest) s940, showed high Notch levels in the midbrain, but, again, no difference in expression at the sites of kugeln (n = 45 kugeln from 19 4 dpf embryos; two experimental repeats).
  5. Kugel number was statistically significantly increased by inhibition of Wnt signalling by 4 h treatment with 10 μm XAV‐939 (***P = 0.0003; control n = 22 embryos 1.32 ± 0.24 (mean ± s.e.m.); XAV‐939 n = 21 embryos 4.29 ± 0.71 (mean ± s.e.m.); 3 dpf; three experimental repeats; Mann–Whitney U‐test).
  6. Kugel diameter was not statistically significantly altered by XAV‐939 (P = 0.4098; control n = 29 kugeln from 22 embryos 8.78 ± 0.76 (mean ± s.e.m.); XAV‐939 n = 90 kugeln from 21 embryos 7.99 ± 0.56 (mean ± s.e.m.); 3 dpf; three experimental repeats; Student's t‐test)
  7. Kugel number was statistically significantly increased by activation of Wnt signalling by 4 h treatment with 10 μm GSK3 inhibition XV (p 0.0359; control n = 22 embryos 1.04 ± 0.34 (mean ± s.e.m.); GSK3 inhibitor n = 21 embryos 4.24 ± 1.28 (mean ± s.e.m.); 3 dpf; three experimental repeats; Mann–Whitney U‐test).
  8. The diameter of kugel was not statistically significantly altered by GSK3 inhibition (p 0.5555; control n = 23 kugeln from 22 embryos 7.26 ± 0.89 (mean ± s.e.m.); GSK3 inhibitor n = 89 kugeln from 21 embryos 8.09 ± 0.97 (mean ± s.e.m.); 3 dpf; three experimental repeats; Student's t‐test).