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. 2015 Apr 9;208(1):137–148. doi: 10.1111/nph.13373

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© 2015 The Authors. New Phytologist © 2015 New Phytologist Trust

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Three‐point cross to define gene order for the S locus, Primula vulgaris Oakleaf and sepaloid. Progeny analysis of a three‐point cross between pin female parents (s/s), homozygous for the wild‐type recessive alleles of oakleaf (okl) and sepaloid (sep), and thrum (S/s) male plants, heterozygous for the dominant Oakleaf (OKL) and recessive sepaloid (sep) alleles, with OAK in coupling with S and sep in coupling with s. Phenotypes and genotypes of different progeny classes are indicated with progeny numbers from each of four crosses shown. The number in each class (Totals) and numbers in each recombination category (Sum totals) are indicated. A total of 601 progeny were characterized.