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. 2015 Apr 9;208(1):137–148. doi: 10.1111/nph.13373

Figure 4.

Figure 4

Mapping Primula vulgaris S locus‐linked genes onto three‐point cross recombinants. Twelve plants (1–12) obtained from the Oakleaf–S locus–Hose in Hose three‐point cross, that represent single‐crossover recombination events between either Oakleaf and the S locus or Hose in Hose and the S locus, are shown. The phenotypes of individuals (O, Oakleaf; P, pin; T, thrum; H, Hose in Hose; wt, wild‐type) are indicated, as well as the location of the crossover in each case in relation to the S locus (S) and the phenotypic markers Oakleaf (O) and Hose in Hose (H). (a) Autoradiograph of gel blot analysis of Hind III‐digested genomic DNA using PvSLL1 as probe. Pin (P)‐ and thrum (T)‐specific alleles of PvSLL1, as revealed by restriction fragment length polymorphisms (RFLPs), are indicated. An inconsistent RFLP obtained from plant 6 is identified by asterisks. (b) Autoradiograph of gel blot analysis of XbaI‐digested genomic DNA using PvSLL2 as probe. P‐ and T‐specific alleles of PvSLL1, as revealed by RFLPs, are indicated. The absence of a thrum‐specific RFLP in plant 9 is identified by asterisks.