Skip to main content
. 2016 Jun 20;38(7):387–402. doi: 10.1111/pim.12326

Figure 3.

Figure 3

Effect of F. hepatica Infection on Recovery of M. bovis and M. bovis BCG‐GFP from MDM. For Experiment 3, MDM were isolated from cattle before, and 4 week and 10 week after F. hepatica infection and then incubated with M. bovis (Mb) (a) or BCG‐GFP (b) (MOI 1 : 1) in vitro for 24 h or added to MDM previously stimulated with FhES (ES) (20 μg/mL) for 24 h. Cultures in 7H11‐OADC agar plates of mycobacteria show that 10 weeks post‐F. hepatica infection, M. bovis recovery per macrophage was lower than before F. hepatica infection, although this was not statistically significant until after co‐stimulation with FhES (*=P < 0.05) (a). Similarly, lower numbers of BCG‐GFP were recovered 10 weeks post‐F. hepatica infection either with or without FhES restimulation (*=P < 0.05) (b). The CFU per macrophage were calculated, with three replicates per animal (represented as different symbols). Horizontal lines represent the mean CFU per macrophage ± SEM, n = 6.