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. 2015 Jan 14;13(9):1251–1263. doi: 10.1111/pbi.12314

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© 2015 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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2D‐PAGE separation profiles of proteins extracted from 4 days after flowering (DAF) grains of (a, b) Yukinkomai, (c, d) Yukinosei and (e, f) Todorokiwase grown under (a, c, e) normal or (b, d, f) heat stress treatment. Protein extracts were separated by isoelectric focusing followed by SDS‐PAGE. Proteins identified included heat shock proteins 70 (HSP70; 66 kDa, pI 4.8), 20S proteasome αF (46 kDa, pI 5.7), protein disulphide isomerase (PDI; 40 kDa, pI 5.3), ABA‐inducible protein (R40g2; 38 kDa, pI 6.8), ascorbate peroxidase (APX; 36 kDa, pI 5.2), alcohol dehydrogenase (ADH; 28 kDa, pI 5.0), Mn superoxide dismutase 1 (MSD1; 28 kDa, pI 5.6), HSP16.9 (18 kDa, pI 5.6) were identified in the 2D‐gels.