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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: J Am Soc Mass Spectrom. 2019 Feb 25;30(5):855–863. doi: 10.1007/s13361-019-02143-4

Figure 2.

Figure 2.

Observed isotopic distributions of c3 (A) and y9 (C) fragment ions from nCID of the natural and deuterated peptide P1 (HHHHHHIIKIIK). The weighted average m/z value for each species is illustrated by a solid green (natural) or grey (deuterated) line. Theoretical deuteration levels for 100 and 0% scrambling are denoted with dashed orange (100%) and blue (0%) lines, respectively. Deuteration levels for all measured P1 nCID-derived c- (B) and y-type (D) fragment ions. Blue and orange lines represent expected deuteration levels for 0 and 100% scrambling, respectively.