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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Cell Signal. 2018 May 21;49:30–38. doi: 10.1016/j.cellsig.2018.05.008

Figure 5.

Figure 5.

Ephrin-A1-induced NFAT1 activation and VCAM-1 expression require calcium/calcineurin signaling. A) HAECs were treated with BAPTA (5 mM), cyclosporine A (CsA, 3 μM), or FK506 (10 nM) for 1 hour prior to treatment with dimerized ephrin-A1-Fc (2 μg/ml). Nuclear NFAT accumulation was assessed by subcellular fractionation and Western blotting for NFAT1; n = 4–6 B) Treatment with BAPTA (5 mM, 1 hour) blunts ephrin-A1-induced VCAM-1 expression as assessed by Western blotting; n = 7. Statistical comparisons were made with 2-way ANOVA with Bonferroni posttest.