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. 2019 Aug;189(8):1621–1636. doi: 10.1016/j.ajpath.2019.04.010

Figure 2.

Figure 2

Confirmation of presynaptic and post-synaptic elements of synaptosomes. A: Human cortical P-2 samples were immunolabeled for the presynaptic vesicular protein synaptophysin and the post-synaptic scaffold protein postsynaptic density-95 (PSD-95). Image is from an ImageStream multispectral imaging flow cytometer, with 25,000 events collected. B: Identically labeled samples analyzed on a BD-FACS Calibur analytic flow cytometer; forward scatter (FSC) is proportional to size. Rectangular analysis gate defines positive fraction on the basis of isotype control. Scale bar = 7 μm (A).