Figure 7.

Restoration of endothelial forkhead box protein M1 (Foxm1) expression in Hif1a^f/f/Tie2Cre⁺ (CKO) mice normalizes endothelial cell (EC) proliferation. A: Representative micrographs of immunostaining of lung sections. At 12 hours after cecal ligation and puncture (CLP), Hif1a^f/f/Tie2Cre⁺ mice were administered intravenously with liposome/Foxm1 plasmid DNA (CKO + Foxm1) or vector (CKO + Vector). Wild-type (WT) mice were also administered with vector. At 72 hours after CLP, mouse lungs were collected. Cryosections were immunostained with anti–5-bromo-2-deoxyuridine (BrdU) (green) and anti-CD31/anti–von Willebrand factor (vWF; red) antibodies. Nuclei were counterstained with DAPI (blue). B: Quantification of BrdU-positive ECs and non-ECs. C and D: Real-time quantitative RT-PCR analysis of expression of genes regulating cell cycle progression. Data are expressed as means ± SD (B–D). ^∗∗P < 0.01, ^∗∗∗P < 0.001 (t-test). Scale bar = 50 μm (A).