Figure 1.

Example pipeline for the production of a 2D DNA structure from a mesh. A) First, an arbitrarily sized mesh (gray) is created in a 3D graphics software program. From this canvas, the desired mesh (red) is produced by deleting the extra edges and vertices. B) The edges and vertices of the mesh can then be remodeled to create the desired shape. C) An uninterrupted scaffold path through the mesh is then found algorithmically. D) A physical simulation where the DNA helices are represented by rigid cylinders connected by springs is used to generate a DNA model with minimized internal tension. E) This DNA model is imported to vHelix where the final DNA staple design and sequences are generated.