Table 1.

Clinical characteristics of EAE induced mice

Parameters	Vehicle	20 mg/kg/d	40 mg/kg/d	80 mg/kg/d	p
Incidence rate 1	100%	100%	100%	100%	–
Day of onset	6.30 ± 0.37	9.00 ± 0.58**	8.90 ± 0.48***	9.33 ± 1.14*	0.011
Time of peak score	13.40 ± 1.13	20.56 ± 2.21*	13.10 ± 2.05	17.89 ± 2.62	0.038
Peak score	2.20 ± 0.13	3.11 ± 0.48*	1.50 ± 0.17	1.67 ± 0.17	0.001
Cumulative score	54.50 ± 3.12	29.89 ± 3.90***	24.20 ± 3.48***	24.89 ± 3.62***	<0.001

Statistical analysis was performed using one‐way anova followed by LSD post hoc test. Values were shown as mean ± SEM. Statistical significance: *p < 0.05, compared with vehicle‐treated mice; **p < 0.01, compared with vehicle‐treated mice; ***p < 0.001, compared with vehicle‐treated mice.

¹Definition of successful induction of EAE: clinicalscore ≥ 1 was observed in neurological assessment with in 35 days.