Figure 2. MALAT1 regulates cardiomyocytes apoptosis in HL-1 cells.

Cells were subjected to 10 h of hypoxia with 95% N₂, 5% CO₂, and subsequent 2 h of reoxygenation with 95% O₂, 5% CO₂; (A) Total RNAs were extracted from control (Ctrl) and H/R group were reverse transcribed into cDNA. Then qRT-PCR was performed to examine the relative expression of MALAT1. (B) HL-1 cells were transfected with pcDNA-MALAT1 or shMALAT1, and then the expression levels of MALAT1 were examined by qRT-PCR. (C) Western blot assay was performed to examine the cleaved caspase 3 and cleaved PARP in in control, H/R cell, H/R cell transfected with MALAT1-pcDNA and shMALAT1 and the expression levels of c-caspase3 and c-PARP were quantified. (D) Annexin V and PI double staining was conducted in control, H/R cell, H/R cell transfected with MALAT1-pcDNA and shMALAT1 and then flow cytometry assay was performed to determine the cell apoptosis. The experiments were repeated for at least three times. n=5, *P<0.05; **P<0.01.