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. 2019 Aug 2;39(8):BSR20191103. doi: 10.1042/BSR20191103

Figure 5. The effect of MALAT1 on cardiomyocytes apoptosis was mediated by miR-144.

Figure 5

(A) Mice injected with shMALAT1 followed with I/R injury. MiR-144 mRNA levels in the indicated each group was examined by qRT-PCR. (B) The cleaved caspase3 and PARP in each group were examined by Western blot assay in HL-1 cells. (C) Annexin V and PI double staining were performed then the cell apoptosis rate in each group were determined and analyzed by flow cytometry in HL-1 cells. (D) HL-1 cells were transfected miR-144-3p mimic, MALATA-pcDNA or miR-1444 together with MALAT1, and then Western blot was performed to check the protein expression of cleaved caspase3 and PARP. All the results presented the data from three independent experiments with the similar results. n=5, *P<0.05; **P<0.01.