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. 2019 Jul 16;11(7):342. doi: 10.3390/pharmaceutics11070342

Figure 5.

Figure 5

The gating strategy of CD4+, CD8+ T cell and germinal center B cell in spleen and MLN cells. Spleen and MLN cells from mice (n = 5) were collected at day 30 upon challenge infection with T. gondii ME49 and stained with phenotype-specific marker antibodies (CD3, CD4, CD8, B220, GL7). CD4+, CD8+ T cell gating strategy in spleen cells (A). CD4+, CD8+ T cell gating strategy in MLN cells (B). Germinal center B cell gating strategy in spleen cells (C). Germinal center B cell gating strategy in MLN cells (D).