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. 2019 Jun 28;11(7):1097. doi: 10.3390/polym11071097

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) schematic depicting the lysozyme/bPE micelle formation and the lysozyme cargos releasing into the cells. Also shown are the hydrodynamic sizes, zeta potentials and size polydispersity of the lysozyme/bPE micelles at various bPE/lysozyme ratios; (b) analysis of penetration ability of crosslinked lysozyme/bPE micelles in multicellular tumor spheroids using light-sheet microscopy. (A) FITC-labelled lysozyme (green), (B) Cy5-labelled CEA polymers (red), (C) Overlay of (A) and (B) showing respective distributions of polymers and lysozymes (D) Fluorescence intensity showing the relative distribution of polymer and lysozyme in the area marked with white box in (C). Adapted from Ref. [51] with permission from the Royal Society of Chemistry.